Coding

Part:BBa_K3190108

Designed by: Ojas Tulsyan   Group: iGEM19_UCopenhagen   (2019-10-13)


Xenopus laevis lutropin-choriogonadotropic hormone receptor LHCGR CDS tagged with myc

Mammalian Luteinizing Hormones (LH) share structural similarity, functional equivalency, and bind the same receptor as hCG; this suggests that Xenopus LHCGR may serve as a good alternative to Homo sapiens LHCGR for the detection of the ligand i.e. luteinizing hormone as LH has been found to induce maturation of Xenopus oocytes in vitro (Wlizla et al., 2017). The gene encoding for the receptor was codon optimised, tagged with myc tag in the C-terminus of the receptor and coupled to the strongest constitutive promoter pCCW12 for heterologous expression in S. cerevisiae, in order to confirm and characterize the protein expression.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 428
    Illegal BglII site found at 1682
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None