Coding

Part:BBa_K3190105

Designed by: Ojas Tulsyan   Group: iGEM19_UCopenhagen   (2019-10-13)


Homo sapiens luteinizing hormone/choriogonadotropin receptor (LHCGR), CDS

The luteinizing hormone/choriogonadotropin receptor (LHCGR), also lutropin/choriogonadotropin receptor (LCGR) or luteinizing hormone receptor (LHR) is a transmembrane receptor found predominantly in the ovary and testis, but also many extragonadal organs such as the uterus and breasts. The receptor interacts with both luteinizing hormone (LH) and chorionic gonadotropins (such as hCG in humans) and represents a G protein-coupled receptor (GPCR). Its activation is necessary for the hormonal functioning during reproduction. The gene for the LHCGR is found on chromosome 2 p21 in humans, close to the FSH receptor gene. The gene encoding for the receptor was codon optimised tagged with myc and coupled to the strongest constitutive promoter pCCW12 for heterologous expression in S. cerevisiae, wherein we expect to characterize the protein expression.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1643
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 145
    Illegal NgoMIV site found at 1324
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1558


[edit]
Categories
Parameters
None