Composite

Part:BBa_K3112046

Designed by: Cheng Chen   Group: iGEM19_Tianjin   (2019-10-21)


Chromosomal stabilization element group example 3

We tested the related functions of chromosomal stabilization element group in Schizosaccharomyces pombe, which is far away from Saccharomyces cerevisiae. We chose a location far away from the peripheral heterochromatin of the original cells. Considering the need for the cell itself to propagate, we did not destroy its peripheral heterochromatin structure and avoided the kinetochore binding region.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 166
    Illegal PstI site found at 518
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 166
    Illegal PstI site found at 518
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 166
    Illegal PstI site found at 518
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 166
    Illegal PstI site found at 518
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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