Composite

Part:BBa_K3112045

Designed by: Cheng Chen   Group: iGEM19_Tianjin   (2019-10-14)


PGK1P+A to G adenine Base Editor+PGK1T

It is a fusion protein consisting of a rigid linker between cytidine deaminase and dcas9. It can be precisely edited at specific locations on target sequences guided by gRNA.

We added promoters and terminators to it and integrated them into plasmids for expression.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 995
    Illegal PstI site found at 1044
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1044
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 995
    Illegal PstI site found at 1044
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 995
    Illegal PstI site found at 1044
    Illegal NgoMIV site found at 2972
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None