Composite

Part:BBa_K3112040

Designed by: Cheng Chen   Group: iGEM19_Tianjin   (2019-10-14)


Chromosomal stabilization element group example 0

Our chromosome stabilizing elements consist of two parts: centromeres and tag for screening.

The insertion site of this element is about 10K in the protocentromere of Yarrowia lipolytica, without excessive damage to its peripheral heterochromatin structure.

Before fusion, the whole set of elements will not have a great impact on the growth and development of cells, it only plays its role in the fusion system. It can stabilize this chromosome with stable elements when the other side of the fusion system is dominant.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 750
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1837
    Illegal PstI site found at 750
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1728
    Illegal BglII site found at 1788
    Illegal BamHI site found at 846
    Illegal XhoI site found at 2642
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 750
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 750
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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