DNA

Part:BBa_K3112028

Designed by: Cheng Chen   Group: iGEM19_Tianjin   (2019-10-14)


Homologous arm in Saccharomyces cerevisiae V Chromosome 1

This is a segment located very close to the centromere of the V chromosome of Saccharomyces cerevisiae.It is used to integrate Yarrowia lipolytica centromeres with the tag into Saccharomyces cerevisiae's genome and replace its original centromeres.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 279
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 279
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 279
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 279
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 279
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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