Coding

Part:BBa_K3112023

Designed by: Cheng Chen   Group: iGEM19_Tianjin   (2019-10-14)


APOBEC1 cytidine deaminase

A part of A to G Adenine Base Editor. APOBEC1 cytidine deaminase is a kind of cytidine deaminase expressed in Escherichia coli.It converts adenine into inosine irreversibly.We optimized this part of the codon to make it more suitable for Saccharomyces cerevisiae.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 289
    Illegal PstI site found at 338
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 338
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 289
    Illegal PstI site found at 338
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 289
    Illegal PstI site found at 338
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None