Coding

Part:BBa_K3112021

Designed by: Cheng Chen   Group: iGEM19_Tianjin   (2019-10-14)


A to G adenine Base Editor

It is a fusion protein consisting of a rigid linker between cytidine deaminase and dcas9. It can be precisely edited at specific locations on target sequences guided by gRNA.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 289
    Illegal PstI site found at 338
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 338
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 289
    Illegal PstI site found at 338
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 289
    Illegal PstI site found at 338
    Illegal NgoMIV site found at 2266
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None