Coding

Part:BBa_K3112020

Designed by: Cheng Chen   Group: iGEM19_Tianjin   (2019-10-14)

Hygromycin Resistance Gene (hyg or hph)

Hygromycin B is an aminoglycosidic antibiotic that inhibits protein synthesis by disrupting translocation and promoting mistranslation at the 80S ribosome. Because it uses a different mode of action than Gibco Geneticin, Blasticidin S, or Gibco Zeocin, it’s perfect for dual-selection experiments when used in conjunction with another selection agent.It is also widely used in yeast.



Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 238
    Illegal PstI site found at 328
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 238
    Illegal PstI site found at 328
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 238
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 238
    Illegal PstI site found at 328
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 238
    Illegal PstI site found at 328
  • 1000
    COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None