Composite

Part:BBa_K3054025

Designed by: Dharmik Hitesh Patel   Group: iGEM19_Chalmers-Gothenburg   (2019-10-20)


bphA4 + pTDH3/pCIT2 + bphA3

The plasmid vector we used had a terminator for yeast in it. Hence we did not add a terminator to the composite. However, adding a terminator to this biobrick should be okay since it has a stop codon in it


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 2933
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 2933
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1914
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 2933
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 2933
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None