DNA

Part:BBa_K3046033

Designed by: Philip Srensen   Group: iGEM19_DTU-Denmark   (2019-10-21)


IS2 downstream sequence for A. niger genome integration

This part is a long sequence of DNA that are homologous to a part of the albA conidial pigment gene. This homology can be used for homologous recombination of genomic constructs flanked by this IS2 sequence (along with the upstream sequence BBa_K3046032).

Usage and Biology

Characterization

We have demonstrated that the IS2 up- and downstream sequences work as expected qualitatively speaking. We did this by transforming a GFP reporter into Aspergillus niger ATCC1015, and as shown in figure 1, we were able to make the transformants express GFP as opposed to the wildtype control.

The two upper pictures show Aspergillus niger ATCC1015 transformed with the pPEA2P1 plasmid. The two pictures in the bottom show the same fungi, transformed with a self replicating AMA1 plasmid containing the PyrG marker



Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 118
    Illegal PstI site found at 1262
    Illegal PstI site found at 1863
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 118
    Illegal PstI site found at 1262
    Illegal PstI site found at 1863
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 118
    Illegal BglII site found at 1360
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 118
    Illegal PstI site found at 1262
    Illegal PstI site found at 1863
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 118
    Illegal PstI site found at 1262
    Illegal PstI site found at 1863
    Illegal NgoMIV site found at 1250
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1751
    Illegal SapI site found at 2060


[edit]
Categories
Parameters
None