DNA

Part:BBa_K3046032

Designed by: Philip Srensen   Group: iGEM19_DTU-Denmark   (2019-10-21)


IS2 upstream sequence for A.niger genome integration

This part is a long sequence of DNA that are homologous to a part of the albA conidial pigment gene. This homology can be used for homologous recombination of genomic constructs flanked by this IS2 sequence (along with the downstream sequence BBa_K3046033).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1858
    Illegal XbaI site found at 1623
    Illegal SpeI site found at 1097
    Illegal PstI site found at 893
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1858
    Illegal SpeI site found at 1097
    Illegal PstI site found at 893
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1858
    Illegal BamHI site found at 1729
    Illegal XhoI site found at 548
    Illegal XhoI site found at 735
    Illegal XhoI site found at 963
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1858
    Illegal XbaI site found at 1623
    Illegal SpeI site found at 1097
    Illegal PstI site found at 893
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1858
    Illegal XbaI site found at 1623
    Illegal SpeI site found at 1097
    Illegal PstI site found at 893
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None