Measurement

Part:BBa_K3046021

Designed by: Philip Srensen   Group: iGEM19_DTU-Denmark   (2019-10-21)


Promoter test plasmid pAMA1-zing_Pyr This plasmid is designed as a shuttle vector to test different fungal promoters

Usage and Biology

The pAMA1-zing-PyrG plasmid is able to replicate autonomously in Aspergillus niger due to its AMA1 sequence (BBa_K3046024).


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3078
    Illegal EcoRI site found at 5355
    Illegal EcoRI site found at 9415
    Illegal XbaI site found at 1257
    Illegal XbaI site found at 2023
    Illegal XbaI site found at 2505
    Illegal XbaI site found at 3273
    Illegal XbaI site found at 5160
    Illegal XbaI site found at 5928
    Illegal XbaI site found at 6410
    Illegal SpeI site found at 1440
    Illegal SpeI site found at 1451
    Illegal SpeI site found at 1815
    Illegal SpeI site found at 1947
    Illegal SpeI site found at 2071
    Illegal SpeI site found at 2178
    Illegal SpeI site found at 2189
    Illegal PstI site found at 4222
    Illegal PstI site found at 10253
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3078
    Illegal EcoRI site found at 5355
    Illegal EcoRI site found at 9415
    Illegal SpeI site found at 1440
    Illegal SpeI site found at 1451
    Illegal SpeI site found at 1815
    Illegal SpeI site found at 1947
    Illegal SpeI site found at 2071
    Illegal SpeI site found at 2178
    Illegal SpeI site found at 2189
    Illegal PstI site found at 4222
    Illegal PstI site found at 10253
    Illegal NotI site found at 1296
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3078
    Illegal EcoRI site found at 5355
    Illegal EcoRI site found at 9415
    Illegal BglII site found at 7082
    Illegal BglII site found at 9054
    Illegal BamHI site found at 9314
    Illegal BamHI site found at 9440
    Illegal BamHI site found at 9991
    Illegal XhoI site found at 3619
    Illegal XhoI site found at 4336
    Illegal XhoI site found at 4814
    Illegal XhoI site found at 9851
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3078
    Illegal EcoRI site found at 5355
    Illegal EcoRI site found at 9415
    Illegal XbaI site found at 1257
    Illegal XbaI site found at 2023
    Illegal XbaI site found at 2505
    Illegal XbaI site found at 3273
    Illegal XbaI site found at 5160
    Illegal XbaI site found at 5928
    Illegal XbaI site found at 6410
    Illegal SpeI site found at 1440
    Illegal SpeI site found at 1451
    Illegal SpeI site found at 1815
    Illegal SpeI site found at 1947
    Illegal SpeI site found at 2071
    Illegal SpeI site found at 2178
    Illegal SpeI site found at 2189
    Illegal PstI site found at 4222
    Illegal PstI site found at 10253
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3078
    Illegal EcoRI site found at 5355
    Illegal EcoRI site found at 9415
    Illegal XbaI site found at 1257
    Illegal XbaI site found at 2023
    Illegal XbaI site found at 2505
    Illegal XbaI site found at 3273
    Illegal XbaI site found at 5160
    Illegal XbaI site found at 5928
    Illegal XbaI site found at 6410
    Illegal SpeI site found at 1440
    Illegal SpeI site found at 1451
    Illegal SpeI site found at 1815
    Illegal SpeI site found at 1947
    Illegal SpeI site found at 2071
    Illegal SpeI site found at 2178
    Illegal SpeI site found at 2189
    Illegal PstI site found at 4222
    Illegal PstI site found at 10253
    Illegal NgoMIV site found at 4356
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 6949
    Illegal BsaI site found at 8128
    Illegal BsaI.rc site found at 1484
    Illegal BsaI.rc site found at 7902


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