Composite

Part:BBa_K3032083

Designed by: Denis Baronas   Group: iGEM19_Vilnius-Lithuania   (2019-10-16)


LexA75_Q-PAS1 repressor under strong Anderson (1) promoter (Ready for Expression)

This is a ready to use composite part of LexA75_Q-PAS1 repressor under strong Anderson promoter (BBa_J23100) and strong RBS (BBa_B0034).

LexA75_Q-PAS1 repressor is a fusion protein which consists of truncated LexA domain (from Bacillus subtilis) and Q-PAS1 (from Rhodopseudomonas palustris). This repressor binds to promoter containing LexA DNA binding sequence and dissociates when it is illuminated with NIR light. Bphp1 (BBa_K3032016) and ho1 (BBa_K3032015) proteins required for system to work. In total 6 fusion variants were designed where LexA domain varied in length.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 659
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None