Composite

Part:BBa_K3032082

Designed by: Denis Baronas   Group: iGEM19_Vilnius-Lithuania   (2019-10-16)


Gal4_Q-PAS1 repressor under strong Anderson (1) promoter (Ready for Expression)

This is a ready to use composite part of Gal4_Q-PAS1 repressor under strong Anderson promoter (BBa_J23100) and strong RBS (BBa_B0034).

Gal4_Q-PAS1 repressor is a fusion protein which consists of Gal4 domain (from Saccharomyces cerevisiae) and Q-PAS1 (from Rhodopseudomonas palustris). This repressor binds to promoter containing Gal4 DNA binding sequence and dissociates when it is illuminated with NIR light. Bphp1 (BBa_K3032016) and ho1 (BBa_K3032015) proteins required for system to work.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 638
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None