Device

Part:BBa_K3032075

Designed by: Denis Baronas   Group: iGEM19_Vilnius-Lithuania   (2019-10-15)


Ready to use mRFP1-CarH device (medium)

This is a ready to use device based on the combination of BBa_K3032070 and BBa_K3032072 parts, where BBa_K3032070 part is the mRFP1 reporter under light-inducible CarH promoter and strong RBS (BBa_B0034) and BBa_K3032072 part is a CarH repressor under strong medium promoter (BBa_J23110) and strong RBS (BBa_B0034). Cobalamin must present in media for CarH oligomerization.

CarH has cobalamine (Vitamin B12), which has a photosensitive Co-C bond, as a prosthetic group. In the dark, CarH is a tetramer that binds strongly to DNA and represses protein translation. When excited by green light, the photosensitive Co-C bond breaks, this disrupts the tetramer structure, and then gene expression can start.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1712
    Illegal AgeI site found at 1824
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None