Coding

Part:BBa_K3032019

Designed by: Denis Baronas   Group: iGEM19_Vilnius-Lithuania   (2019-10-13)


LexA90_Q-PAS1 repressor

LexA90_Q-PAS1 repressor is a fusion protein which consists of truncated LexA domain (from Bacillus subtilis) and Q-PAS1 (from Rhodopseudomonas palustris). This repressor binds to promoter containing LexA DNA binding sequence and dissociates when it is illuminated with NIR light. Bphp1 (BBa_K3032016) and ho1 (BBa_K3032015) proteins required for system to work. In total 6 fusion variants were designed where LexA domain varied in length.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 643
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 268
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None