Part:BBa_K3031017

Iron QS system + ORF81 antigen protein

This system is designed to express downstream genes of interest only in low iron environments. The LuxI/LuxR QS system ensures only expression of proteins once a high cell density is reached. We have used the modified LuxI promoter designed by this team to regulate expression of downstream genes using a Ferric Uptake Regulator repressor. The QS system is now inhibited as long as ferric iron is available to the cell as LuxI protein is not produced, resulting in no AHL + LuxR complex forming. Therefore the Lux promoter (pLux) is not activated.

Activation in the system is achieved in low iron environments such as the guts of fish. This makes the system ideal for an oral vaccine delivery system where antigen production could be controlled until the cell reaches the gut of the fish after ingestion.

This system was used to express the antigenic protein ORF81 - a membrane envelope protein from the Koi Herpes Virus known to produce an immune response in koi fish.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 2402
  Illegal AgeI site found at 2301
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 985
  Illegal BsaI.rc site found at 1907
  Illegal BsaI.rc site found at 2522
  Illegal SapI site found at 2491