![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K3031007
Designed by: David Doyle Group: iGEM19_SUIS_Shanghai (2019-10-13)
Signalling Peptide Lp_2578 + ORF81 + Cell Wall Anchor
This part contains a rbs (part BBa_) sequence followed by the sequence for Signalling peptide Lp_2578 (part BBa_K3031003) which is flanked by two restriction sites (NdeI and Spel) creating a signalling peptide cassette which can be easily exchanged with another to test the efficiency of exporting of heterlogous proteins in lactobacillus spp.
The protein to be exported or secreted gene of interest can be fused to the end of this sequence. Another signalling peptide can easily replace the SP by digestion and ligation for later experiments into SP efficiency.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 713
Illegal AgeI site found at 612 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 833
Illegal SapI site found at 802
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Categories
Parameters
None |