Reporter

Part:BBa_K3026010

Designed by: Pascale Lemieux   Group: iGEM19_ULaval   (2019-10-16)


Toehold norovirus ORF2 VP1

ToeHold switch targeting mRNA sequence of the VP1 gene from norovirus GII/Hu/JP/2007/GII.P15_GII.15/Sapporo/HK299. This part is flanked by Gibson homology sites for plasmid pBluescript for insertion in cloning site. This part, when expressed in a cell or media containing T7 polymerase, will produce a ToeHold switch with a recognition sequence in the mRNA sequence of the VP1 gene. When this ToeHold is in presence of this mRNA sequence, the sfGFP will be produced. This system allows for detection of RNA fragments or plasmids containing this cassette. For in vivo expression, use strain BL21 DE3.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 110
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 167


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