Reporter

Part:BBa_K3026002

Designed by: Francois D Rouleau   Group: iGEM19_ULaval   (2019-09-08)


ToeHold_DNA_FtsK

ToeHold switch targeting DNA sequence of E. coli ftsK gene. This part is flanked by Gibson homology sites for plasmid pBluescript for insertion in cloning site. This part, when expressed in a cell or media containing T7 polymerase, will produce a ToeHold switch with a recognition sequence in the DNA sequence of the the ftsK essential gene. When this ToeHold is in presence of this DNA sequence, the sfGFP will be produced. This system allows for detection of DNA fragments plasmids or strains containing this gene. For in vivo expression, use strain BL21 DE3.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 168


[edit]
Categories
Parameters
None