Composite
Part:BBa_K3003018
Designed by: Ali Göktuğ Attar Group: iGEM19_Bilkent-UNAMBG (2019-10-16)
TEV Protease in Ag43 Cassette
Tobacco etch virus (TEV) protease is a site-specific protease. In this part, we placed TEV protease in Ag43 cassette to display it on the cell surface. This part can be used to cleave other peptides displayed on the cell surface who has specific TEV recognition site. TEV protease was expressed with low, mid and high copy level to characterize how it cuts the protein of interest from the specific site. System was characterized by the growth curve of the cell and under an inducible promoter, how protein of interest is cut was shown.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 280
Illegal PstI site found at 1021
Illegal PstI site found at 1579
Illegal PstI site found at 2179
Illegal PstI site found at 2803
Illegal PstI site found at 3074
Illegal PstI site found at 3127 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 280
Illegal PstI site found at 1021
Illegal PstI site found at 1579
Illegal PstI site found at 2179
Illegal PstI site found at 2803
Illegal PstI site found at 3074
Illegal PstI site found at 3127 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 280
Illegal PstI site found at 1021
Illegal PstI site found at 1579
Illegal PstI site found at 2179
Illegal PstI site found at 2803
Illegal PstI site found at 3074
Illegal PstI site found at 3127 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 280
Illegal PstI site found at 1021
Illegal PstI site found at 1579
Illegal PstI site found at 2179
Illegal PstI site found at 2803
Illegal PstI site found at 3074
Illegal PstI site found at 3127
Illegal NgoMIV site found at 54
Illegal NgoMIV site found at 847
Illegal NgoMIV site found at 2071
Illegal NgoMIV site found at 2581
Illegal NgoMIV site found at 2602
Illegal AgeI site found at 1597
Illegal AgeI site found at 2341
Illegal AgeI site found at 2755
Illegal AgeI site found at 2997 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |