Part:BBa_K2984014

6x His-Tag for protein detection, isolation, purification B5-B5

This part is a part of the Chlamy-HUB-Collection. The His-Tag is an amino acid motif at the C- or N-terminus of proteins. It is one of the most commonly used purification tags with a minimum of six or more consecutive histidine residues. They are able to form complexes with metal ions like Ni2⁺ or Co2⁺ making it possible to purify the proteins attached to the his-tag when those transition metal ions are connected on beads. The amino acid sequence is HHHHHH.

This part was designed to be used with the MoClo standard and has B5-B5 overhangs.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Characterization

Fig.1 - Successful transformation of L0-6xHis-Tag_B5-B5 into E. coli.