Tag

Part:BBa_K2958003

Designed by: Angelica Sabandal   Group: iGEM19_ULaVerne_Collab   (2019-07-24)


6GGS His Tag 6GGS

6 his tag with a 6 GGS sequence before and after the 6 his tag sequence (6 GGS - 6 his tag - g GGS)

Description:

A his tag (6X His) is a purification tag typically found at the C or N Terminus of a protein.Because this his tag is in the middle of the protein sequence, we have added a 6GGS (Glycine-Glycine-Serine) linker based on the iGEM17_Sydney_Australia part BBa_K2417013 to improve the flexibility of the his tag, and increase the likelihood of it being properly exposed for protein purification. This tag is useful for purification tags that need to be placed in the middle of a sequence, rather than at the C or N terminus, due to construct design.


T--ULaVerne_Collab--InsulinPurificationProcess.png
Figure 1. A step by step diagram of the insulin purification process.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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