Composite

Part:BBa_K2937000

Designed by: Ruochen Liu   Group: iGEM19_RDFZ-China   (2019-10-21)


[lacIQ counst. promoter+lacI coding sequence]reverse [ptac+rbs]forward

The part is designed for those backbones lack the coding of lac operon which is targrting to most of the backbones in iGEM part registry, for IPTG-inducing gene expression. The part includes coding sequence of lacI, lacI Q counstitutive promoter with RBS, for a counstitutive expression of lacI, and also ptac promoter with rbs. lacI is counstitutively produced for repression of ptac promoter, and when lacI inducer is added, lacI-IPTG complex is formed, which inhibits the repress from lacI molecule to ptac, thus downstream coding sequence can be expressed. This part is a improved version of BBa_K18000


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None