Part:BBa_K2908670

pLN431-G8p-yCD-miR101BS

The CSU_CHINA iGEM team 2019 designed a fusion protein consisting of yCD and miR101-BS for sequence-specific expression of yCD .Therefore, we used our miR101-BD(BBa_K2908668) to bind different concentrations of miR101 so that regulate the transactivation domain of yCD(BBa_K2908667).CD converts the prodrug 5-FC (used to treat infections by fungi such as Candida albicans and Cryptococcus neoformans) into the active agent 5-fluorouracil (5-FU). While 5-FC is nontoxic to human cells because of the lack of CD, 5-FU is used to treat cancers like colon, pancreatic, and breast cancer. The cytotoxic effects of 5-FU occur following its conversion to 5-fluoro-2'-deoxyuridine-5'-monophosphate (5-FdUMP). 5-FdUMP is an irreversible inhibitor of thymidylate synthase and thus inhibits DNA synthesis by deoxythymidine triphosphate (dTTP) deprivation and causes DNA strand breakage, leading to cell death. Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 197
  Illegal NheI site found at 1143
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 244
  Illegal XhoI site found at 60
  Illegal XhoI site found at 174
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 755
  Illegal BsaI.rc site found at 792