Part:BBa_K2809002

Prepro-alpha-factor (codon modified for Pichia pastoris)

Prepro-alpha-factor is the secretion signal peptide from the yeast α-mating factor. This signal peptide directs secreted expression of the recombinant protein.In other words, it allows the exportation of it. Once the recombinant protein is in the endoplasmic reticulum(ER) lumen, this secretion signal will be cleaved.

UM_Macau 2021's Contribution

Characterization

The literature published by JH Bae and his team introduced a fusion partner that can enhance the secretion rate of this part[1]. According to this literature, the scientist inserted a modified HL peptide right after the MF alpha-factor to promote secretion. The results indicated that the modified HL 28 peptide can efficiently increase the secretion rate when working with the MF alpha pre-pro peptide. This fusion partner also includes an affinity chromatography tag, which will convince the future purification step of the proteins. The amino acid sequence of HL28: EDEDGDDEYATEETLSHHHHHHGDDDDK

Figure 1. HL28 usage example

Reference

[1] Bae, Jung-Hoon et al. “A novel fusion partner for enhanced secretion of recombinant proteins in Saccharomyces cerevisiae.” Applied microbiology and biotechnology vol. 100,24 (2016): 10453-10461. doi:10.1007/s00253-016-7722-2

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]