Part:BBa_K2782012
Fluorescent protein indicator system with fatty acid operon as the core
Biodiesel refers to a fatty acid methyl ester or ethyl ester formed by the conversion of vegetable oil, animal oil, waste oil or microbial oil with methanol or ethanol. Biodiesel is a typical "green energy" with good environmental performance, good engine start-up performance, good fuel performance, wide range of raw materials, and renewable characteristics. Vigorously developing biodiesel has important strategic significance for sustainable economic development, promoting energy substitution, reducing environmental pressure, and controlling urban air pollution. The entire reaction process in biodiesel production, glycerol removal, catalyst removal, alcohol removal, and ensuring free fatty acids are important. We are submitting a newly designed fatty acid operon that acts as a molecular switch that signals fatty acids to regulate the expression of subsequent genes. In the absence of fatty acids, it binds to repressor proteins, RNA polymerase cannot bind to the promoter sequence, or The RNA polymerase can not move forward along the DNA, repress the transcription, and then the gene can not be expressed. The appropriate amount of fatty acid is added, and the repressor protein binds to the fatty acid, and the gene can be expressed after being detached from the operon. The more fatty acid, the more the fatty acid can be bound. The more repressor proteins, the better the gene expression thereafter. Using fatty acid operons, we can add a reporter gene such as a fluorescent protein gene to the fatty acid operon to obtain a bacteria that can report fatty acid content. The content of fluorescent protein can reflect the level of fatty acid in the oil. Judging the acidity of fats and oils can be used as an indicator to judge the freshness of fats and oils. Similarly, when it is used in biodiesel, it can be used to judge the progress of methyl esterification of biodiesel raw materials into biodiesel, and the detection of free fatty acids in the quality test of finished biodiesel is compared with the original alkali neutralization. The determination of acidity has the advantage of eliminating the interference of acids other than fatty acids, which is of great significance for promoting the development of biodiesel and further precision determination of its quality.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 4
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1400
Illegal AgeI site found at 1512 - 1000COMPATIBLE WITH RFC[1000]
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