Regulatory

Part:BBa_K2782011

Designed by: Bing Gao   Group: iGEM18_BUCT-China   (2018-08-16)


Glyoxylic acid operon（Negative control induction）

The glyoxylate operon is a DNA fragment, and the glyoxylic acid operon designed by the team belongs to the negative control induction system of vector management. The glyoxylic acid operon is loaded into the vector plasmid and transformed into cells, which can achieve a regulatory effect, and the substrate is induced to be glyoxylic acid. When the outside is induced by glyoxylic acid, glyoxylic acid binds to the repressor protein, and the repressor protein is detached, so that transcription starts.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 854
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 555
    Illegal AgeI site found at 1553
    Illegal AgeI site found at 1665
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 654


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Categories
Parameters
None