Regulatory

Part:BBa_K2782005

Designed by: Dan Zhou   Group: iGEM18_BUCT-China   (2018-08-15)


Promoter sequence, sigma factor and RNA polymerase binding site

A promoter is a DNA sequence that RNA polymerase recognizes, binds to, and initiates transcription, and it contains a conserved sequence required for RNA polymerase specific binding and transcription initiation, and the promoter itself is not transcribed. The promoter is typically located upstream of the transcription start site. The promoter part we submitted is after the fatty acid operon. When the signaling molecule fatty acid appears, it binds to the repressor protein, causing the repressor to detach from the operon. The sigma factor and RNA polymerase can recognize the position on the promoter. Point to start transcription.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None