Part:BBa_K2782004
This part can express red fluorescent protein
A reporter gene is a concept of molecular biology, which refers to a class of genes that are expressed in cells, tissues/organs, or individuals under specific conditions and that produce traits that are easy to detect and that are not originally produced by the experimental material. As a reporter gene, there must be several conditions in genetic selection and screening detection: 1 has been cloned and the entire sequence has been determined; 2 the expression product is not present in the recipient cell, ie no background, in the transfected cell There is no similar endogenous expression product; 3 its expression product can be quantitatively determined. The fluorescent protein family is a homologous protein with a relative molecular mass of 20,000 to 30,000 found in the genus and corals. Red fluorescent protein (RFP) is a photoprotein (drFP583 or DsRed) isolated from coral (Discosoma sp.) that emits bright red fluorescence. These commonly used reporter genes can also be used in combination to detect the expression of two or even three genes. The choice of reporter gene depends on its sensitivity, reliability, and dynamic range of monitoring. The well-stabilized reporter gene is suitable for gene transcriptional kinetics research and high-throughput screening, especially for qualitative research of gene transfer.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 555
Illegal AgeI site found at 667 - 1000COMPATIBLE WITH RFC[1000]
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