Protein_Domain

Part:BBa_K2772018

Designed by: Yan Xie   Group: iGEM18_DLUT_China_B   (2018-10-08)


Formylglycine-generating enzyme

FGE can specifically identify ald-Tag,which is consisted of 6 base pair or 13 base pair,then it oxidze sulfhydryl group of sulfhydryl group to aldehyde group.Conveniently, it can be used for in vivo catalysis of the substrate label of the precursor, ie higher conversion can be achieved without purification separation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 367
    Illegal AgeI site found at 723
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None