Part:BBa_K2760005

Promoter spaCBA for Lactobacillus rhamnosus GG

This promoter is part of the SpaCBA operon in Lactobacillus rhamnosus GG, it is not considered a pathogenic factor, as it is presumed to act as niche-adaptation factors in non-pathogenic lactic acid bacteria. L. rhanmosus GG is considered a probiotic strain for the human species. L. rhamnosus's pili are encoded by the SpaCBA Operon, which itself contains genes for three pilin-proteins (spaC, spaB, and spaA) and one pilin-specific sortase (srtC1). The SpaCBA promoter undergoes constitutive expression, unlike other fimbrial spaFED operon in the same study, for the same species [1]. This promoter is meant to be used for constitutive expression in L. rhamnosus GG, and could possibly be tested in other Lactobacillus strains such as L. casei [1].

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 38
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]