Composite

Part:BBa_K2737013

Designed by: Xinyu Teng   Group: iGEM18_ECUST   (2018-10-12)


entBACEDF

It is a cluster to synthesis entrobactin from E.Coli controlled by a quorum-sensing promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 924
    Illegal EcoRI site found at 5328
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 924
    Illegal EcoRI site found at 5328
    Illegal NheI site found at 35
    Illegal NheI site found at 58
    Illegal NheI site found at 4591
    Illegal NheI site found at 4614
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 924
    Illegal EcoRI site found at 5328
    Illegal BglII site found at 4946
    Illegal BamHI site found at 5891
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 924
    Illegal EcoRI site found at 5328
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 924
    Illegal EcoRI site found at 5328
    Illegal AgeI site found at 5739
    Illegal AgeI site found at 7317
    Illegal AgeI site found at 7430
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/biosynthesis
Parameters
None