DNA

Part:BBa_K2644106

Designed by: Xinyi Wang   Group: iGEM18_TJU_China   (2018-07-26)


T7crRNAEGFP（lbCas12a）

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Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage

Due to the diversity of mutation sites in human genes, not all mutation sites have suitable PAM (TTTN). However, Cas12a protein needs PAM to perform recognition function, so the detection of mutation sites is not completely detectable. Moreover, most mutation sites are very close to each other, and a crRNA can only detect one mutation. Considering different mutation sites in human genes correspond to different diseases, the detection is expensive. It is necessary to achieve one-time multi-locus mutation detection as efficiently as possible.

Reference

Li, Shi Yuan, et al. "CRISPR-Cas12a has both cis - and trans -cleavage activities on single-stranded DNA." Cell Research (2018).

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