Composite

Part:BBa_K2636003

Designed by: Sandro Papais   Group: iGEM18_Bordeaux   (2018-10-14)

Aldh1 under strong Promoter and strong RBS


Aldehyde dehydrogenase 1 ( Aldh1 ) catalyses the oxidation of 5-(hydroxyméthyl)furfural ( HMF ) into 2,5-Furandicarboxylic acid ( FDCA ).[1] The regluation of this coding sequence by strong Promoter and RBS allows the transcription and translation at high level for E. coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 380
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 298
    Illegal NgoMIV site found at 616
    Illegal NgoMIV site found at 707
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 659


References

  1. ↑ Hossain, G. S., Yuan, H., Li, J., Shin, H., Wang, M., Du, G., … Liu, L. (2017). crossm Metabolic Engineering of Raoultella, 83(1), 1–15.
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