Coding

Part:BBa_K2636001

Designed by: Sandro Papais   Group: iGEM18_Bordeaux   (2018-10-12)

hmfH oxidoreductase ( hmfH )


hmfH oxidoreductase ( hmfH ) catalyses the oxidation of 5-(hydroxyméthyl)furfural ( HMF ) into 2,5-Furandicarboxylic acid ( FDCA ).[1]


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 84
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 52
    Illegal NgoMIV site found at 746
    Illegal NgoMIV site found at 763
    Illegal NgoMIV site found at 784
    Illegal AgeI site found at 191
    Illegal AgeI site found at 1538
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 673
    Illegal BsaI.rc site found at 1196
    Illegal SapI site found at 841


References

  1. ↑ Koopman, F., Wierckx, N., de Winde, J. H., & Ruijssenaars, H. J. (2010). Efficient whole-cell biotransformation of 5-(hydroxymethyl)furfural into FDCA, 2,5-furandicarboxylic acid. Bioresource Technology, 101(16), 6291–6296. https://doi.org/10.1016/j.biortech.2010.03.050
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