Regulatory
Part:BBa_K2622012
Designed by: Auksė Gaižauskaitė Group: iGEM18_Vilnius-Lithuania (2018-10-10)
RNA thermoswitch3 (GJ6)
RNA thermoswitch3 (GJ6) Low temperatures keep the mRNA at a conformation that masks the ribosome binding site within the 5’ end untranslated region (UTR). Masking of the Shine-Dalgarno (SD) sequence restricts ribosome binding and subsequent protein-translation.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 9
- 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 9
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 9
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 9
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |