Regulatory

Part:BBa_K2622011

Designed by: Auksė Gaižauskaitė   Group: iGEM18_Vilnius-Lithuania   (2018-10-10)


RNA thermoswitch2 (GJ3-)

RNA thermoswitch2 (GJ3) Low temperatures keep the mRNA at a conformation that masks the ribosome binding site within the 5’ end untranslated region (UTR). Masking of the Shine-Dalgarno (SD) sequence restricts ribosome binding and subsequent protein-translation.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 13
    Illegal PstI site found at 19
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 13
    Illegal PstI site found at 19
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 13
    Illegal PstI site found at 19
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 13
    Illegal PstI site found at 19
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None