Coding

Part:BBa_K2574000

Designed by: Jonathan Tello   Group: iGEM18_Hawaii   (2018-10-09)


VLP forming Gag Construct from Maize CR element.

The 2018 Hawaii iGEM team designed a DNA sequence that codes for the proteins necessary for the formation of Virus-Like Particles. Gag; the structure forming unit of retroviral and retroviral-like elements, is further divided into the matrix, capsid, and nucleocapsid domains. The make-up and function of Gag domains varies widely between retroviral and retroviral-like elements. Our design derives from a Maize centromeric retrotransposon (CR) element. Our sequence also contains a Histidine tag at the 5' end which is used in DNA purification using a nickel based column.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None