Regulatory

Part:BBa_K2569021

Designed by: Dongyang Dong   Group: iGEM18_SKLMT-China   (2018-09-29)

Pfme, constitutive promoter in Pseudomonas. fluorescence pf-5

Promoter Pfme is taken from the upstream region of fem,whose length is 70bp. It is one of the constitutive promoters of Pseudomonas. fluorescence promoter library,its strength is characterized by a reporter gene ,firefly luciferase. It can be used to regulate the gene expression in Pseudomonadaceae through 3A Assembly.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 63
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 63
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 63
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 63
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 63
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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