Regulatory

Part:BBa_K2569006

Designed by: wenxue Zhao   Group: iGEM18_SKLMT-China   (2018-09-27)


PfliA, constitutive promoter in Pseudomonas. fluorescence pf-5

Promoter PfliA is taken from the upstream region of fliA, whose length is 70bp.It is one of the constitutive promoters of pseudomonas fluorescence promoter library,its strength is characterized by a reporter gene, firefly luciferase.It can be used to regulate the gene expression in Pseudomonadaceae through 3A Assembly.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 62
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None