Composite

Part:BBa_K2562004

Designed by: Lauren Hartz   Group: iGEM18_ASIJ_Tokyo   (2018-09-30)


SERPINA1 with E342K point mutation using an OsmY tag, a GFP reporter system, and a double terminator

The SERPINA1 gene coding for the Alpha-1 Antitrypsin protein is derived from Homo sapiens.The SERPINA1 coding sequence has a point mutation at E342K, a replacement of glutamic acid for lysine. The mutation accounts for Alpha-1 antitrypsin deficiency, in which the lack of Alpha-1 antitrypsin leads to a defective lung and liver. Its secretion was optimized through an OsmY tag and reported through GFP fluorescence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1256
    Illegal NheI site found at 1279
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 209
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1934


[edit]
Categories
//awards/composite_part
//plasmid/expression/t7
Parameters
None