DNA

Part:BBa_K2542008

Designed by: Yaoqiang Zheng   Group: iGEM18_NEFU_China   (2018-10-05)


Part of the transposable element of Saccharomyces cerevisiae.

It' a part of the transposable element of Saccharomyces cerevisiae, which can be used to perform homologous recombination of DNA to insert the sequence of interest into the genome of Saccharomyces cerevisiae. The other is BBa_K2542007.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Experiment

We placed the homology arms (BBa_K2542007 & BBa_K2542008) at both ends of the insertion sequence.Then we extracted the yeast genome and used PCR to test whether the homologous recombination was successful.

T--NEFU China--homologous recombination PCR.jpg
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Categories
Parameters
None