Part:BBa_K2534022

TetR

[http://2018.igem.org/Team:AFCM-Egypt# Team:AFCM-Egypt 2018] created BBa K2534022 tetracycline Resistance gene as an efficient switching control system for lentiviral delivery of mIRNAs into Colorectal cancer cell line RKO. This comes in a trial to to improve tetR registry part BBa_K182005 which lacks a terminator We also worked to improve this part by adding a universal terminator To improve the control of the highly specific interaction of the tet repressor protein (TetR) with (tetO). TetR binding on tetO classically stops expression of genes downstream of tetO by excluding RNA polymerase from binding the promoter and initiating transcription. [http://2018.igem.org/Team:AFCM-Egypt# Team:AFCM-Egypt 2018] have characterized this part considering it's construction into the transfer vector of the lentiviral delivery.

For further information about demonstration of our results, please visit our results page http://2018.igem.org/Team:AFCM-Egypt/Demonstrate For further information about design of our parts, please visit our Parts & Design Page http://2018.igem.org/Team:AFCM-Egypt/Parts

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]