Coding

Part:BBa_K2510016

Designed by: Alicia Graham   Group: iGEM17_Paris_Bettencourt   (2017-10-27)


PhaA and PhaB under T3 Promoter and PhaC under K1F Promoter

PhaA and PhaB under the T3 promoter and with a double terminator with PhaC . These are the three enzymes in PH3B production pathway. Separating them under two different promoters allows the creation of logic gates. This part lacks the terminator at the end, found in the design of BBa_K2510060.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 597
    Illegal BglII site found at 1422
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 574
    Illegal NgoMIV site found at 886
    Illegal NgoMIV site found at 1165
    Illegal NgoMIV site found at 2550
    Illegal NgoMIV site found at 2691
    Illegal NgoMIV site found at 2858
    Illegal NgoMIV site found at 3704
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3772


[edit]
Categories
Parameters
None