Composite

Part:BBa_K2476003

Designed by: Vivian Hu   Group: iGEM17_Pittsburgh   (2017-10-27)


Mutant CheY regulated by IPTG

Uses an IPTG inducible promoter with RBS (BBa_J04500), Mut CheY coding region (BBa_K2476011), and a double terminator (BBa_B0015). Will express a constitutively active Mutant (YD13K Y106W) CheY protein when induced with IPTG.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 590
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None