Coding

Part:BBa_K2455001

Designed by: Jon Fugl   Group: iGEM17_UCopenhagen   (2017-10-20)


His-tagged TrpE gene from E. coli MG1655

TrpE gene from E. coli strain MG1655 carrying an M293T point mutation (to avoid negative feedback inhibition) and a hexa histidine-tag attached by a short linker sequence.

TrpE codes for the Anthranilate enzyme responsible for the first step of the conversion of Chorismate into to tryptophan.

Previous work (Gu et al., 2012) have shown that overexpression of TrpE leads to increased tryptophan production in E. coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 965
    Illegal BglII site found at 1026
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/biosynthesis
//cds/enzyme
//chassis/prokaryote/ecoli
//direction/forward
Parameters
directionForward
functionSynthesis
originEscherichia coli MG1655
proteinEnzyme