Part:BBa_K2455000

His-tagged AroG gene from E. coli MG1655

AroG gene from E. coli strain MG1655 carrying an P150L point mutation (to avoid negative feedback inhibition) and a hexa histidine-tag attached by a short linker sequence.

AroG is the first enzyme of the shikimate pathway leading to synthesis of chorismate, which subsequently can be converted to tryptophan, tyrosine, and phenylalanine. Being the first enzyme in the pathway, it determines the carbon flow towards amino acid synthesis and thus the production.

Previous work (Gu et al., 2012) have shown that overexpression of AroG leads to increased tryptophan production in E. coli.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 934
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 241