Composite

Part:BBa_K2449031

Designed by: Sarah Jrgensen   Group: iGEM17_SDU-Denmark   (2017-10-25)


RelB controlled by indicuble pBAD/AraC promoter

The antitoxin RelB from the RelE-RelB toxin-antitoxin system under control of the pBAD/AraC promoter. Using this part, the expression of RelB can be regulated manually. Addition of arabinose induces expression from this promoter, whereas addition of glucose has an inhibitory effect.
The manual regulation of this part was simulated using a reporter system, see experience on part BBa_I0500 and BBa_I6058.


Sequence and Features
The intermediate part without the promoter can be found in part BBa_K2449028


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal XhoI site found at 1339
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


[edit]
Categories
Parameters
None